論文：松崎友祐, 他 (2015) 20種類の精油におけるCandida albicansに対する抗真菌活性. アロマテラピー学雑誌15（1）:78-87.

Abstract: Fungi belonging to the genus Candida are responsible for opportunistic infectious diseases including oral candidiasis, which causes oral pain. In the case of the elderly who require nursing care, the adherence of C. albicans to denture surfaces is considered to be the cause of the symptoms. We examined whether essential oils could be used as cleaning agents of C. albicans-infected dentures. We selected 20 kinds of essential oils from three different manufactures, and two surfactants, Tween 80 and Tween 20. The antifungal activities of the 60 essential oils in total were studied under three conditions: addition of Tween 80, Tween 20, and no surfactant. The evaluation was made based on minimal inhibitory concentration (MIC) values determined by standardized antibiotic susceptibility testing. After the confirmation of MIC values, the minimal fungicidal concentration (MFC) values were also examined. More than half of the essential oils were fungicidally active without surfactants; however, the number was increased with the addition of surfactants, exhibiting the fungicidal activities at lower concentrations. Among the oils tested, lemongrass showed the lowest MIC, with a concentration less than 0.016%. The fungicidal activities in some essential oils of the same species varied among manufacturers. It was also found that Tween 20 was more effective than Tween 80 at amplifying the fungicidal activities of essential oils at lower concentrations. The results of this study provide evidence that some essential oils are effective as new denture-cleaning agents. In selecting essential oils for cleansing agents, it is important to identify the most effective oil types, even of the same name or species, and to choose the most suitable surfactant.
Key words: essential oil, oral candidiasis, Candida albicans, Tween 80, Tween 20